أضخم موقع علمي صحي موثق في العالم لسعادة العائلة وصحتها, بالعلم والإيمان نبني الميزان

88056119

The isolation and identification of vitamin D2 and vitamin D3from Medicago sativa (alfalfa plant).

The isolation and identification of vitamin D2 and vitamin D3from Medicago sativa (alfalfa plant).



 

         
  No.  Divisions/Titles for Abstract Details

 
  1 Abstract Title The isolation and identification of vitamin D2 and vitamin D3from Medicago sativa (alfalfa plant).  
  2 Abstract Source Archives of Biochemistry and Biophysics, 231, 67-71.  
  3 Abstract Author(s) Horst, R. L., Reinhardt, T. A., Russel, J. R. & Napoli, J. L.   
  4 Article Affiliation National Animal Disease Center, Agricultural Research Service, U. S. Department of Agriculture, Ames, Iowa 50010 U.S.A.  
  5 Abstract Vitamin D2 (Ergocalciferol, also known as vitamin D₂ and calciferol, is a type of vitamin D found in food and used as a dietary supplement. As a supplement it is used to prevent and treat vitamin D deficiency)and vitamin D3 (Cholecalciferol, also known as vitamin D₃ and colecalciferol, is a type of vitamin D which is made by the skin, found in some foods, and taken as a dietary supplement)were isolated from Medicago sativa (alfalfa) grown under field and laboratory conditions and then irradiated with ultraviolet light. The vitamins were identified by ultraviolet absorption,(refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. This means it uses light in the visible and adjacent ranges) mass spectroscopy,( is an analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio. In simpler terms, a mass spectrum measures the masses within a sample. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures.) and comparison with synthetic standards on several chromatographic systems. Sun-cured, field-grown alfalfa contained vitamin D2 at a concentration of 48 ng/g (1920 IU/kg) and vitamin D3 at 0.63 ng/g (25 IU/kg). Laboratory-grown alfalfa, artificially irradiated, contained vitamin D2 at a concentration of 80 ng/g and vitamin D3 at 1.0 ng/g. Therefore, the presence of vitamin D2, as well as vitamin D3, has unequivocally been demonstrated in alfalfa plant tissue.  
  6 Summary Vitamin D2 and vitamin D3 were isolated from Medicago sativa (alfalfa) grown under field and laboratory conditions and then irradiated with ultraviolet light. The vitamins were identified by ultraviolet absorption, mass spectroscopy, and comparison with synthetic standards on several chromatographic systems. Sun-cured, field-grown alfalfa contained vitamin D2 at a concentration of 48 ng/g (1920 IU/kg) and vitamin D3 at 0.63 ng/g (25 IU/kg). Laboratory-grown alfalfa, artificially irradiated, contained vitamin D2 at a concentration of 80 ng/g and vitamin D3 at 1.0 ng/g. Therefore, the presence of vitamin D2, as well as vitamin D3, has unequivocally been demonstrated in alfalfa plant tissue.  
  7 Article Published Date 15-may-1984  
  8
Study Type invitro  
  9
Substances alfalfa  
  10
Diseases constituent study  
  11 Pharmacological Actions vitamins  
  12
Link https://goo.gl/AxAeZv  
       

 


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